dna methylation Search Results


ez dna methylation lightning kit  (Zymo Research)
99
Zymo Research ez dna methylation lightning kit
Ez Dna Methylation Lightning Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ez dna methylation lightning kit - by Bioz Stars, 2026-09
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bisulfite conversion  (Vazyme Biotech Co)
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Vazyme Biotech Co bisulfite conversion
Bisulfite Conversion, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+methylation/EpiArt+DNA+Methylation+Library+Kit+for+Illumina+V3/pm41786706-396-44-49
Average 94 stars, based on 1 article reviews
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ez dna methylation gold kit zymo research  (Zymo Research)
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Zymo Research ez dna methylation gold kit zymo research
Ez Dna Methylation Gold Kit Zymo Research, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+methylation/EZ+DNA+Methylation-Gold+Kit/pm41928513-236-45-49
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ez 96 dna methylation kit  (Zymo Research)
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Zymo Research ez 96 dna methylation kit
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ez 96 dna methylation kit - by Bioz Stars, 2026-09
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global dna methylation line 1 kit  (Active Motif)
95
Active Motif global dna methylation line 1 kit
Global Dna Methylation Line 1 Kit, supplied by Active Motif, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+methylation/Global+DNA+Methylation+-+LINE-1+Kit/pmc13006433-413-7-13
Average 95 stars, based on 1 article reviews
global dna methylation line 1 kit - by Bioz Stars, 2026-09
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human genomic dna  (EpigenDx)
94
EpigenDx human genomic dna
Primary human monocyte-derived macrophages (hMDM) from sixteen donors were treated with dopamine (10 -6 M) for 3 hours with or without pre-treatment using hypomethylating agents 5-aza-2’-deoxycytidine (dAZA; 10 -6 M), 5-azacytidine (AZA; 10 -6 M), or vehicle control. RNA and <t>DNA</t> were isolated for analysis of IL-1β gene expression by qPCR and IL-1β DNA methylation, respectively. (A) Dopamine increased IL-1β mRNA expression, an effect inhibited by dAZA (n= 7-8 donors, *p<0.05). (B) AZA pretreatment produced a similar inhibitory effect on dopamine-induced IL-1β expression, trending toward statistical significance (n=5-6, p=0.0513). (C) Dopamine treatment significantly <t>increased</t> <t>methylated</t> IL-1β levels compared to vehicle control (n=16, p<0.05). (D) Lipopolysaccharide (LPS) increased IL-1β DNA methylation; however, this effect did not reach statistical significance in this donor cohort (n=16). (E) LPS-induced changes in IL-1β DNA methylation were significantly correlated with dopamine-induced changes within the same donors, indicating consistent donor-specific responsiveness across stimuli (n=16, **p<0.01).
Human Genomic Dna, supplied by EpigenDx, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+methylation/Human+High+Methylated+DNA/bio_rxiv__64898__2026__01__21__700899-78-4-11
Average 94 stars, based on 1 article reviews
human genomic dna - by Bioz Stars, 2026-09
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low methylated dna  (EpigenDx)
94
EpigenDx low methylated dna
Primary human monocyte-derived macrophages (hMDM) from sixteen donors were treated with dopamine (10 -6 M) for 3 hours with or without pre-treatment using hypomethylating agents 5-aza-2’-deoxycytidine (dAZA; 10 -6 M), 5-azacytidine (AZA; 10 -6 M), or vehicle control. RNA and <t>DNA</t> were isolated for analysis of IL-1β gene expression by qPCR and IL-1β DNA methylation, respectively. (A) Dopamine increased IL-1β mRNA expression, an effect inhibited by dAZA (n= 7-8 donors, *p<0.05). (B) AZA pretreatment produced a similar inhibitory effect on dopamine-induced IL-1β expression, trending toward statistical significance (n=5-6, p=0.0513). (C) Dopamine treatment significantly <t>increased</t> <t>methylated</t> IL-1β levels compared to vehicle control (n=16, p<0.05). (D) Lipopolysaccharide (LPS) increased IL-1β DNA methylation; however, this effect did not reach statistical significance in this donor cohort (n=16). (E) LPS-induced changes in IL-1β DNA methylation were significantly correlated with dopamine-induced changes within the same donors, indicating consistent donor-specific responsiveness across stimuli (n=16, **p<0.01).
Low Methylated Dna, supplied by EpigenDx, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+methylation/Human+Low+Methylated+DNA/pmc06006560-187-3-6
Average 94 stars, based on 1 article reviews
low methylated dna - by Bioz Stars, 2026-09
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ez dna methylation kit  (Zymo Research)
99
Zymo Research ez dna methylation kit
Primary human monocyte-derived macrophages (hMDM) from sixteen donors were treated with dopamine (10 -6 M) for 3 hours with or without pre-treatment using hypomethylating agents 5-aza-2’-deoxycytidine (dAZA; 10 -6 M), 5-azacytidine (AZA; 10 -6 M), or vehicle control. RNA and <t>DNA</t> were isolated for analysis of IL-1β gene expression by qPCR and IL-1β DNA methylation, respectively. (A) Dopamine increased IL-1β mRNA expression, an effect inhibited by dAZA (n= 7-8 donors, *p<0.05). (B) AZA pretreatment produced a similar inhibitory effect on dopamine-induced IL-1β expression, trending toward statistical significance (n=5-6, p=0.0513). (C) Dopamine treatment significantly <t>increased</t> <t>methylated</t> IL-1β levels compared to vehicle control (n=16, p<0.05). (D) Lipopolysaccharide (LPS) increased IL-1β DNA methylation; however, this effect did not reach statistical significance in this donor cohort (n=16). (E) LPS-induced changes in IL-1β DNA methylation were significantly correlated with dopamine-induced changes within the same donors, indicating consistent donor-specific responsiveness across stimuli (n=16, **p<0.01).
Ez Dna Methylation Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+methylation/EZ+DNA+Methylation+Kit/pm41820501-67-12-16
Average 99 stars, based on 1 article reviews
ez dna methylation kit - by Bioz Stars, 2026-09
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human methylated dna  (Zymo Research)
95
Zymo Research human methylated dna
Primary human monocyte-derived macrophages (hMDM) from sixteen donors were treated with dopamine (10 -6 M) for 3 hours with or without pre-treatment using hypomethylating agents 5-aza-2’-deoxycytidine (dAZA; 10 -6 M), 5-azacytidine (AZA; 10 -6 M), or vehicle control. RNA and <t>DNA</t> were isolated for analysis of IL-1β gene expression by qPCR and IL-1β DNA methylation, respectively. (A) Dopamine increased IL-1β mRNA expression, an effect inhibited by dAZA (n= 7-8 donors, *p<0.05). (B) AZA pretreatment produced a similar inhibitory effect on dopamine-induced IL-1β expression, trending toward statistical significance (n=5-6, p=0.0513). (C) Dopamine treatment significantly <t>increased</t> <t>methylated</t> IL-1β levels compared to vehicle control (n=16, p<0.05). (D) Lipopolysaccharide (LPS) increased IL-1β DNA methylation; however, this effect did not reach statistical significance in this donor cohort (n=16). (E) LPS-induced changes in IL-1β DNA methylation were significantly correlated with dopamine-induced changes within the same donors, indicating consistent donor-specific responsiveness across stimuli (n=16, **p<0.01).
Human Methylated Dna, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+methylation/Universal+Methylated+Human+DNA+Standard/pm41871060-70-0-3
Average 95 stars, based on 1 article reviews
human methylated dna - by Bioz Stars, 2026-09
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high methylated genomic dna  (EpigenDx)
94
EpigenDx high methylated genomic dna
Primary human monocyte-derived macrophages (hMDM) from sixteen donors were treated with dopamine (10 -6 M) for 3 hours with or without pre-treatment using hypomethylating agents 5-aza-2’-deoxycytidine (dAZA; 10 -6 M), 5-azacytidine (AZA; 10 -6 M), or vehicle control. RNA and <t>DNA</t> were isolated for analysis of IL-1β gene expression by qPCR and IL-1β DNA methylation, respectively. (A) Dopamine increased IL-1β mRNA expression, an effect inhibited by dAZA (n= 7-8 donors, *p<0.05). (B) AZA pretreatment produced a similar inhibitory effect on dopamine-induced IL-1β expression, trending toward statistical significance (n=5-6, p=0.0513). (C) Dopamine treatment significantly <t>increased</t> <t>methylated</t> IL-1β levels compared to vehicle control (n=16, p<0.05). (D) Lipopolysaccharide (LPS) increased IL-1β DNA methylation; however, this effect did not reach statistical significance in this donor cohort (n=16). (E) LPS-induced changes in IL-1β DNA methylation were significantly correlated with dopamine-induced changes within the same donors, indicating consistent donor-specific responsiveness across stimuli (n=16, **p<0.01).
High Methylated Genomic Dna, supplied by EpigenDx, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+methylation/Monkey+High+Methylated+DNA/pmc07725942-254-26-30
Average 94 stars, based on 1 article reviews
high methylated genomic dna - by Bioz Stars, 2026-09
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ez dna methylation  (Zymo Research)
96
Zymo Research ez dna methylation
Primary human monocyte-derived macrophages (hMDM) from sixteen donors were treated with dopamine (10 -6 M) for 3 hours with or without pre-treatment using hypomethylating agents 5-aza-2’-deoxycytidine (dAZA; 10 -6 M), 5-azacytidine (AZA; 10 -6 M), or vehicle control. RNA and <t>DNA</t> were isolated for analysis of IL-1β gene expression by qPCR and IL-1β DNA methylation, respectively. (A) Dopamine increased IL-1β mRNA expression, an effect inhibited by dAZA (n= 7-8 donors, *p<0.05). (B) AZA pretreatment produced a similar inhibitory effect on dopamine-induced IL-1β expression, trending toward statistical significance (n=5-6, p=0.0513). (C) Dopamine treatment significantly <t>increased</t> <t>methylated</t> IL-1β levels compared to vehicle control (n=16, p<0.05). (D) Lipopolysaccharide (LPS) increased IL-1β DNA methylation; however, this effect did not reach statistical significance in this donor cohort (n=16). (E) LPS-induced changes in IL-1β DNA methylation were significantly correlated with dopamine-induced changes within the same donors, indicating consistent donor-specific responsiveness across stimuli (n=16, **p<0.01).
Ez Dna Methylation, supplied by Zymo Research, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+methylation/EZ+DNA+Methylation-Direct+Kit/10__1016_slash_j__isci__2026__115475-581-12-17
Average 96 stars, based on 1 article reviews
ez dna methylation - by Bioz Stars, 2026-09
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epimark methylated dna enrichment kit  (New England Biolabs)
95
New England Biolabs epimark methylated dna enrichment kit
Primary human monocyte-derived macrophages (hMDM) from sixteen donors were treated with dopamine (10 -6 M) for 3 hours with or without pre-treatment using hypomethylating agents 5-aza-2’-deoxycytidine (dAZA; 10 -6 M), 5-azacytidine (AZA; 10 -6 M), or vehicle control. RNA and <t>DNA</t> were isolated for analysis of IL-1β gene expression by qPCR and IL-1β DNA methylation, respectively. (A) Dopamine increased IL-1β mRNA expression, an effect inhibited by dAZA (n= 7-8 donors, *p<0.05). (B) AZA pretreatment produced a similar inhibitory effect on dopamine-induced IL-1β expression, trending toward statistical significance (n=5-6, p=0.0513). (C) Dopamine treatment significantly <t>increased</t> <t>methylated</t> IL-1β levels compared to vehicle control (n=16, p<0.05). (D) Lipopolysaccharide (LPS) increased IL-1β DNA methylation; however, this effect did not reach statistical significance in this donor cohort (n=16). (E) LPS-induced changes in IL-1β DNA methylation were significantly correlated with dopamine-induced changes within the same donors, indicating consistent donor-specific responsiveness across stimuli (n=16, **p<0.01).
Epimark Methylated Dna Enrichment Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+methylation/EpiMark+Methylated+DNA+Enrichment+Kit/pm41792463-202-11-16
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Image Search Results


Primary human monocyte-derived macrophages (hMDM) from sixteen donors were treated with dopamine (10 -6 M) for 3 hours with or without pre-treatment using hypomethylating agents 5-aza-2’-deoxycytidine (dAZA; 10 -6 M), 5-azacytidine (AZA; 10 -6 M), or vehicle control. RNA and DNA were isolated for analysis of IL-1β gene expression by qPCR and IL-1β DNA methylation, respectively. (A) Dopamine increased IL-1β mRNA expression, an effect inhibited by dAZA (n= 7-8 donors, *p<0.05). (B) AZA pretreatment produced a similar inhibitory effect on dopamine-induced IL-1β expression, trending toward statistical significance (n=5-6, p=0.0513). (C) Dopamine treatment significantly increased methylated IL-1β levels compared to vehicle control (n=16, p<0.05). (D) Lipopolysaccharide (LPS) increased IL-1β DNA methylation; however, this effect did not reach statistical significance in this donor cohort (n=16). (E) LPS-induced changes in IL-1β DNA methylation were significantly correlated with dopamine-induced changes within the same donors, indicating consistent donor-specific responsiveness across stimuli (n=16, **p<0.01).

Journal: bioRxiv

Article Title: Epigenetic Regulation of Inflammation by Dopamine in Primary Human Macrophages

doi: 10.64898/2026.01.21.700899

Figure Lengend Snippet: Primary human monocyte-derived macrophages (hMDM) from sixteen donors were treated with dopamine (10 -6 M) for 3 hours with or without pre-treatment using hypomethylating agents 5-aza-2’-deoxycytidine (dAZA; 10 -6 M), 5-azacytidine (AZA; 10 -6 M), or vehicle control. RNA and DNA were isolated for analysis of IL-1β gene expression by qPCR and IL-1β DNA methylation, respectively. (A) Dopamine increased IL-1β mRNA expression, an effect inhibited by dAZA (n= 7-8 donors, *p<0.05). (B) AZA pretreatment produced a similar inhibitory effect on dopamine-induced IL-1β expression, trending toward statistical significance (n=5-6, p=0.0513). (C) Dopamine treatment significantly increased methylated IL-1β levels compared to vehicle control (n=16, p<0.05). (D) Lipopolysaccharide (LPS) increased IL-1β DNA methylation; however, this effect did not reach statistical significance in this donor cohort (n=16). (E) LPS-induced changes in IL-1β DNA methylation were significantly correlated with dopamine-induced changes within the same donors, indicating consistent donor-specific responsiveness across stimuli (n=16, **p<0.01).

Article Snippet: Both high-methylated and low-methylated human genomic DNA (80-8061-HGHM5 and 80-8062-HGUM5 from EpigenDx) were bisulfite treated and used as controls for the primer sets.

Techniques: Derivative Assay, Control, Isolation, Gene Expression, DNA Methylation Assay, Expressing, Produced, Methylation